ELISA is one of the gold standards for in-vitro diagnostic testing. Despite ongoing developments including lab-on-chip, immunosensors, microfluidics, and other point of care technologies ELISA technologies have opened up a lucrative market due to the unmatched reliability and high throughput for monitoring and management of disease markers. In the conventional ELISA, antibodies are coated in the microplates that bind to selected target proteins. Another set of antibodies that bind to another site on the target protein is used for detection for the bound proteins to the pre-coated antibodies forming a sandwich complex. However the one-step ELISA simplifies this process. This is done with the use of semi-homogeneous mixture wherein the sandwich complex is formed in to solution in one step and is anchored to the plate with immunoaffinity tags for identification. The one-step ELISA takes almost half the time required to run the conventional sandwich ELISA.
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Immunoassays are the most common type of assays used in the pharmaceutical industry for routine monitoring to drug and drug impurities. Thus, pharmaceutical industry is the largest end user of the one-step ELISA kits. The one-step ELISA kits can be used for quantifying natural proteins as well as for recombinant proteins. These one-step ELISA kits are commonly used for, monitoring production process, screening of clones, and other similar biotechnology applications.
One-step ELISA Market: Drivers and Restraints
Rising demand for rapid tests, need for simplified testing kits, increased research of biomarkers are some of the factors that drive the growth of the one-step ELISA market globally. However, factors such as lack of awareness, lack of sufficient sensitivity, stringent regulations and the fact that most products for a variety of known biomarkers is yet to be developed or is still being tested for clinical use, are some of the factors that are hindering the growth of the global one-step ELISA market.
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One-step ELISA Market: Segmentation
The global One-step ELISA market is segmented by target, sample type, end users and region:
Based on the target, the global One-step ELISA market is segmented into:
- TNF alpha
Based on the sample type, the global One-step ELISA market is segmented into:
- Serum or Plasma
- Tissue Lysate
Based on the end users, the global One-step ELISA market is segmented into:
- Academic and Research Institutes
- Pharmaceutical and Biotechnology Companies
- Diagnostic Centers
- Hospitals & Clinics
One-step ELISA Market: Overview
One-step ELISA market is segmented into target, sample type, end user and regions. One-step ELISA can be performed to identify a number of targets in humans such as IgG, Albumin, TNF alpha, IL-6, Fibrinogen, Insulin, Lysozyme and others. The One-step ELISA can be performed using a body fluids such as plasma or serum as well as using tissue lysate as sample type for the identification of certain biomarkers. One-step ELISA is being popularly used as a diagnostic tool as well as for identification of presence of biomarkers in drug discovery and development processes. Thus the end users of one-step ELISA include academic and research institutes, pharmaceutical and biotechnology companies, diagnostic centers, hospitals and clinics.
One-step ELISA Market: Regional Outlook
North America and Europe take the maximum share of the One-step ELISA market. Increased adoption of advance technology, rise in healthcare budget, increase in awareness, and increasing demand for quick results in these regions are some of the factors that drive the growth of the One-step ELISA market. However in regions such as China and India, the growing demand for rapid tests, ageing population and limited number of healthcare professionals has created an opportunity for the growth of the One-step ELISA market in such regions. Thus, the One-step ELISA market is expected to grow with a significant CAGR in the APAC region.
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One-step ELISA Market: Key Players
Some of the Key players in the One-step ELISA market include, Abcam plc, Boster Biological Technology, DIALAB GmbH, AnaSpec, Inc., IDS PLC, PerkinElmer, Inc., InBios International, Inc., among many others.